Can SOP2 be connected to VCC permanently?

It says here SOP2 needs VCC to enable UART mode. Can I make that connection permanent or does it need to be disconnected again to actually use the box?

As long as SOP2 is set the Box will boot into UART Mode. This will not start the firmware on the box. So it should only be set during flashing.

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If you do it like me, you can pull SOP2 high with the UART:

When the CP2102 or FT232RL is connected, the SUP2 gets pulled HIGH, so no need for manually set bridges.
Also always append “—reset dtr” to your command (right after defining the port) (e.g. cc3200tool -p COM3 —reset dtr read_file /cert/ca.der cert/ca.der)